Immunoelectron microscopy - Methods and protocols
نویسندگان
چکیده
منابع مشابه
Immunoelectron microscopy of human retroviruses.
The ultrastructural detection and identification of human retroviruses--HTLV (human T-cell lymphotropic virus) and HIV (human immunodeficiency virus)--have become an everyday task for pathologists and virologists as well as for cell and molecular biologists. The development of better and conventionally available immunocytochemical techniques, such as pre- or postembedding immunocytochemical met...
متن کاملImmunoelectron microscopy of aldehyde-fixed yeast cells.
Immunolocalization of antigens in cells requires simultaneous preservation of structure and antigenicity. The difficulties of immunolocalization methods are centered around the methods used to preserve structure, which as a rule tend to degrade the ability of antigens (usually proteins) to be recognized by their cognate antibodies. The value of immunolocalization for cell biology was early reco...
متن کاملCapsule of Escherichia coli K29: ultrastructural preservation and immunoelectron microscopy.
The polysaccharide capsule of Escherichia coli K29 fully surrounds the microorganism and thus occupies an extracellular space ca. 20 times larger in volume than that of the decapsulated cell. Since more than 95% of the capsule consists of water, dehydration for electron microscopy causes the material to collapse. We describe here a method for embedding the capsule in an uncollapsed form. Dehydr...
متن کاملA “non-classical” and reliable method for transmission immunoelectron microscopy
Sample preparation for transmission immunoelectron microscopy techniques is pivotal to preserve cell architecture and to maintain antigen epitopes. We propose a “non-classical” method in order to obtain the above-mentioned conditions. In a pilot series of experiments, we found a mixture of 2.0% paraformaldehyde/0.1% glutaraldehyde in 0.1 M PBS (pH 7.4) to be an optimal fixing solution. In this ...
متن کاملSizing of protein A-colloidal gold probes for immunoelectron microscopy
Gold particles in colloidal solutions often vary considerably in size. The finest sols (diameter less than 15 nm), especially, are very heterogeneous, as is indicated by coefficients of variance (CV) of 25-35%. We have complexed staphylococcal protein A with gold particles (PA/Au) and then fractionated the preparations by glycerol or sucrose gradient centrifugation into very homogeneous subfrac...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: European Journal of Histochemistry
سال: 2011
ISSN: 2038-8306,1121-760X
DOI: 10.4081/ejh.2011.br11